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Image Search Results
Journal: bioRxiv
Article Title: The nuclear sulfenome of Arabidopsis : spotlight on histone acetyltransferase GCN5 regulation through functional thiols
doi: 10.1101/2024.04.24.590918
Figure Lengend Snippet: Predicted structure of Arabidopsis GCN5 and experimental evidence for its oxidative post-translational modification. (A) Schematic diagram of the GCN5 domains and positions of the individual cysteine residues. (B) Multi-domain homology model of GCN5 according to MODELLER. (C) AlphaFold2 prediction of the GCN5 structure. Coloured based on pLDDT score. (D) Sulfenylation of recombinant His/MBP-tagged GCN5 protein upon H 2 O 2 treatment. Recombinant GCN5 protein was incubated with increasing H 2 O 2 concentrations, loaded on a gel and visualised with anti-cysteine sulfenic acid antibody. Ponceau staining shows the loading control. His/MBP-tagged GCN5 migrated as a single band at ∼106 kDa. (E) Histone acetyltransferase activity of recombinant HisMBP-GCN5 in the presence of H 2 O 2.
Article Snippet: A
Techniques: Modification, Recombinant, Incubation, Staining, Activity Assay